PLK1 and RAP1GAP

Number of citations: 21 (PubMedID 25329897)
Data Source: BioGRID (affinity chromatography technology, affinity chromatography technology, enzymatic study)

		PLK1	RAP1GAP
Name		polo like kinase 1	RAP1 GTPase activating protein
Structure
GO Annotations	Cellular Component	Chromosome, Centromeric Region Kinetochore Condensed Chromosome, Centromeric Region Chromatin Synaptonemal Complex Spindle Pole Outer Kinetochore Nucleus Nucleoplasm Chromosome Cytoplasm Centrosome Centriole Spindle Cytosol Cytoskeleton Spindle Microtubule Microtubule Cytoskeleton Midbody Centriolar Satellite Spindle Midzone Mitotic Spindle Pole	Golgi Membrane Cytoplasm Early Endosome Golgi Apparatus Cytosol Membrane Axon Dendrite Neuronal Cell Body
	Molecular Function	Nucleotide Binding Magnesium Ion Binding Protein Kinase Activity Protein Serine/threonine Kinase Activity Protein Binding ATP Binding Microtubule Binding Anaphase-promoting Complex Binding Kinase Activity Transferase Activity Protein Kinase Binding Identical Protein Binding Protein Serine Kinase Activity	GTPase Activity GTPase Activator Activity Protein Binding GTPase Regulator Activity Small GTPase Binding Protein Homodimerization Activity
	Biological Process	Mitotic Sister Chromatid Segregation G2/M Transition Of Mitotic Cell Cycle Negative Regulation Of Transcription By RNA Polymerase II Establishment Of Mitotic Spindle Orientation Mitotic Cell Cycle Nuclear Division Mitotic Cytokinesis Double-strand Break Repair Via Homologous Recombination Microtubule Bundle Formation Double-strand Break Repair Protein Phosphorylation Mitotic Spindle Organization Sister Chromatid Cohesion Mitotic Chromosome Condensation Mitotic Nuclear Membrane Disassembly Metaphase/anaphase Transition Of Mitotic Cell Cycle Mitotic Spindle Assembly Checkpoint Signaling Mitotic G2 DNA Damage Checkpoint Signaling Centrosome Cycle Regulation Of Mitotic Cell Cycle Regulation Of Cell Cycle Process Female Meiosis Chromosome Segregation Protein Ubiquitination Peptidyl-serine Phosphorylation Regulation Of Mitotic Metaphase/anaphase Transition Protein Destabilization Positive Regulation Of Proteasomal Ubiquitin-dependent Protein Catabolic Process Regulation Of Cytokinesis Protein Localization To Organelle Negative Regulation Of Apoptotic Process Homologous Chromosome Segregation Establishment Of Protein Localization Positive Regulation Of Proteolysis Golgi Inheritance Nuclear Membrane Disassembly Centrosome Separation Cell Division Positive Regulation Of Ubiquitin-protein Transferase Activity Regulation Of Cell Cycle Synaptonemal Complex Disassembly Protein Localization To Chromatin Protein Localization To Centrosome Protein Localization To Nuclear Envelope Double-strand Break Repair Via Alternative Nonhomologous End Joining Positive Regulation Of Protein Localization To Nucleus Regulation Of Mitotic Spindle Assembly Regulation Of Mitotic Cell Cycle Phase Transition Positive Regulation Of Ubiquitin Protein Ligase Activity Regulation Of Protein Localization To Cell Cortex Regulation Of Anaphase-promoting Complex-dependent Catabolic Process Protein Localization To Site Of Double-strand Break Negative Regulation Of Double-strand Break Repair Via Homologous Recombination	Adaptive Immune Response Phagocytosis Signal Transduction Positive Regulation Of Cell-cell Adhesion Regulation Of GTPase Activity Positive Regulation Of GTPase Activity Negative Regulation Of Neuron Differentiation Positive Regulation Of Phagocytosis Regulation Of Small GTPase Mediated Signal Transduction Establishment Of Localization In Cell Cell-cell Adhesion Negative Regulation Of Microvillus Assembly Negative Regulation Of Thyroid Gland Epithelial Cell Proliferation Cellular Response To Glial Cell Derived Neurotrophic Factor
Pathways		Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal Polo-like kinase mediated events Golgi Cisternae Pericentriolar Stack Reorganization Golgi Cisternae Pericentriolar Stack Reorganization APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 Phosphorylation of the APC/C Phosphorylation of Emi1 Condensation of Prophase Chromosomes Separation of Sister Chromatids Resolution of Sister Chromatid Cohesion Regulation of PLK1 Activity at G2/M Transition Activation of NIMA Kinases NEK9, NEK6, NEK7 Loss of Nlp from mitotic centrosomes Recruitment of mitotic centrosome proteins and complexes Loss of proteins required for interphase microtubule organization from the centrosome Recruitment of NuMA to mitotic centrosomes Anchoring of the basal body to the plasma membrane RHO GTPases Activate Formins Mitotic Prometaphase Mitotic Metaphase/Anaphase Transition Mitotic Telophase/Cytokinesis Cyclin A/B1/B2 associated events during G2/M transition The role of GTSE1 in G2/M progression after G2 checkpoint AURKA Activation by TPX2 EML4 and NUDC in mitotic spindle formation Regulation of MITF-M-dependent genes involved in cell cycle and proliferation	Rap1 signalling RET signaling
Drugs		3-[3-chloro-5-(5-{[(1S)-1-phenylethyl]amino}isoxazolo[5,4-c]pyridin-3-yl)phenyl]propan-1-ol 3-[3-(3-methyl-6-{[(1S)-1-phenylethyl]amino}-1H-pyrazolo[4,3-c]pyridin-1-yl)phenyl]propanamide 4-(4-METHYLPIPERAZIN-1-YL)-N-[5-(2-THIENYLACETYL)-1,5-DIHYDROPYRROLO[3,4-C]PYRAZOL-3-YL]BENZAMIDE 1-[5-Methyl-2-(trifluoromethyl)furan-3-yl]-3-[5-[2-[[6-(1H-1,2,4-triazol-5-ylamino)pyrimidin-4-yl]amino]ethyl]-1,3-thiazol-2-yl]urea Wortmannin Fostamatinib Volasertib	None
GWAS		Mean corpuscular volume ( 32888494 ) Mean reticulocyte volume ( 32888494 )	Abdominal aortic calcification levels ( 31659325 ) Heel bone mineral density ( 30598549 ) High light scatter reticulocyte count ( 27863252 32888494 ) High light scatter reticulocyte percentage of red cells ( 27863252 ) Immature fraction of reticulocytes ( 27863252 ) Liver enzyme levels ( 18940312 ) Mean reticulocyte volume ( 32888494 ) Metabolite levels ( 23823483 ) Neurocognitive impairment in HIV-1 infection (continuous) ( 28447399 ) Serum alkaline phosphatase levels ( 31666285 )
Interacting Genes		147 interacting genes: - ACTL6B AKAP12 APP ASPM BAG6 BCL2L1 BIRC6 BRCA2 BUB1 C6orf136 CBL CCNB1 CDC14A CDC25C CDC6 CEBPA CENPQ CENPU CEP55 CHEK2 CSN1S1 CSN2 CTNNB1 CUL4B DNAJB9 DNHD1 ECT2 EIF6 ERCC6L EYA1 EYA4 FBXL5 FBXW7 GET4 GLB1 GORASP1 HNRNPU IDH1 IDH2 IKBKB INTS11 ITSN1 KIF23 KIF2C KLF4 KLHL22 KRABD3 LMO4 LRP5L LRRK1 LRRK2 MAD2L1BP MAGED1 MCM2 MCM3 MCM7 MDM2 MISP MPP2 MYC MYT1 NCAPD3 NCAPG2 NCAPH2 NEDD1 NEDD4 NHSL2 NINL NPM1 NUDC PARP10 PHC2 PIN1 PITPNM1 PKMYT1 PON1 PPID PPIL2 PPP1R12A PPP6R2 PRC1 PRKN PSMA1 PSMA3 PSMA4 PSMA5 PSMA6 PSMA7 PSMB1 PSMB2 PSMB3 PSMB4 PSMB5 PSMB6 PSMB7 PSRC1 PTEN PTPRD RAB1A RABAC1 RACGAP1 RAD51 RAP1GAP RECQL5 RELA REST RGCC RICTOR RNF126 RNF2 RSF1 RXRA SEPTIN9 SHCBP1 SIMC1 SNCA SNCB SPOUT1 SREBF1 STAG2 STUB1 SUGT1 TIMELESS TNFSF11 TOP2A TP53 TP53BP2 TP73 TPT1 TRIOBP TSC1 TUBA4A TUBB TUBB3 TUBG1 UBE2I UHRF1 USP16 USP7 VIM VMA22 VRK2 VRK3 WAC WEE1 ZNF71	18 interacting genes: AFDN BRAF CDK1 CSNK1G2 DSCR9 DYNC1I1 GNAZ LCOR MAP1LC3B MAPK6 MEX3B PFN2 PLK1 PRKACA RALGDS RAP1A VPS26C ZBTB12
Entrez ID		5347	5909
HPRD ID		03652	02609
Ensembl ID		ENSG00000166851	ENSG00000076864
Uniprot IDs		P53350	P47736 X6R8W7
PDB IDs		1Q4K 1Q4O 1UMW 2OGQ 2OJX 2OU7 2OWB 2RKU 2V5Q 2YAC 3BZI 3C5L 3FC2 3FVH 3HIH 3HIK 3KB7 3P2W 3P2Z 3P34 3P35 3P36 3P37 3Q1I 3RQ7 3THB 4A4L 4A4O 4DFW 4E67 4E9C 4E9D 4H5X 4H71 4HAB 4HCO 4HY2 4J52 4J53 4LKL 4LKM 4O56 4O6W 4O9W 4RCP 4WHH 4WHK 4WHL 4X9R 4X9V 4X9W 5J19 5NEI 5NFU 5NJE 5NMM 5NN1 5NN2 5TA6 5TA8 6AX4 6GY2 7MSO 7MX1 8BJT 8CRC 8JOQ 8JOY 8S30 8S31 8WFP 8X72 8XB9	1SRQ 3BRW
GO Terms Enriched among Interactors		Proteasome Core Complex Cell Division Cytoplasm Regulation Of Cell Cycle Nucleoplasm Regulation Of Cell Cycle Process Nucleus Cytosol Cellular Response To Stress Ubiquitin-dependent Protein Catabolic Process Modification-dependent Protein Catabolic Process Protein Catabolic Process Proteasome Complex Proteasomal Protein Catabolic Process Proteolysis Involved In Protein Catabolic Process Proteasome-mediated Ubiquitin-dependent Protein Catabolic Process DNA Damage Response Macromolecule Catabolic Process Positive Regulation Of Cell Cycle Proteasome Core Complex, Beta-subunit Complex Positive Regulation Of Cell Cycle Process Regulation Of Mitotic Cell Cycle Spindle Regulation Of Cell Cycle Phase Transition Proteasome Core Complex, Alpha-subunit Complex Regulation Of Cellular Response To Stress Microtubule-based Process Organelle Organization Centrosome Cytoskeleton Microtubule Cytoskeleton Organization Midbody Proteolysis Regulation Of Intracellular Signal Transduction Cellular Response To Radiation DNA Repair Chromosome Segregation Regulation Of Chromosome Organization Regulation Of Cytokinesis Macromolecule Metabolic Process Response To Xenobiotic Stimulus Regulation Of Organelle Organization Regulation Of Mitotic Cell Cycle Phase Transition Positive Regulation Of Chromosome Segregation Regulation Of Apoptotic Signaling Pathway Negative Regulation Of Cell Cycle Positive Regulation Of Programmed Cell Death Protein Kinase Binding Chromosome Regulation Of Chromosome Segregation	Protein Phosphorylation Phosphorylation Protein Serine Kinase Activity Protein Serine/threonine Kinase Activity Protein Kinase Activity Negative Regulation Of Synaptic Vesicle Exocytosis Regulation Of Synaptic Vesicle Exocytosis Kinase Activity Phosphate-containing Compound Metabolic Process Mitotic Nuclear Membrane Disassembly Regulation Of Neurotransmitter Secretion Nuclear Membrane Disassembly Membrane Disassembly Intracellular Signaling Cassette Nucleotide Binding Positive Regulation Of Peptidyl-serine Phosphorylation Regulation Of Regulated Secretory Pathway Regulation Of Secretion By Cell Regulation Of Protein Localization Protein Localization To Site Of Double-strand Break Regulation Of Secretion Regulation Of Peptidyl-serine Phosphorylation Negative Regulation Of Secretion By Cell Postsynaptic Modulation Of Chemical Synaptic Transmission Synaptic Vesicle Exocytosis Positive Regulation Of Protein Modification Process Mitotic G2 DNA Damage Checkpoint Signaling Negative Regulation Of Exocytosis Establishment Of Endothelial Barrier Chromosome Condensation Negative Regulation Of Secretion Positive Regulation Of D-glucose Transmembrane Transport Pronuclear Fusion Spindle Microtubule Endothelial Cell Development Peptidyl-serine Phosphorylation Regulation Of Exocytosis Protein Modification Process Nuclear Membrane Organization Centrosome Cycle Sperm Midpiece Presynapse Intracellular Signal Transduction Postsynapse Positive Regulation Of Actin Filament Bundle Assembly Positive Regulation Of Protein Phosphorylation Modulation Of Chemical Synaptic Transmission MAPK Cascade ERK1 And ERK2 Cascade Positive Regulation Of Stress Fiber Assembly